Qureshi has highlighted three "major navigational concerns" relating to the project, which include whether the Thames will continue to allow two-way traffic along the river.
Other concerns include whether the proposed floating walkway will be able to withstand the impact of a vessel and whether barge access will be secured between Southwark Bridge and Cannon Street.
Qureshi said: "Unfortunately, the mayor was not able to reassure me and the many others who have serious concerns about the safety logistics of this plan."
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